Coding

Part:BBa_K1844001:Design

Designed by: David Lips   Group: iGEM15_Amsterdam   (2015-09-17)


AckA1 coding part


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 729
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 379
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Contained PstI site. Removed via site-directed mutagenesis.


Source

Comes from the genomic sequence of lactococcus lactis.

References